genetic characterization of toxoplasma gondii from zoo wild¬life and pet birds in fujian, china

background: toxoplasmosis, a worldwide zoonotic disease, is caused by toxo­plasma gondii . the distribution of genetic diversity of t. gondii in wild animals is of great importance to understand the transmission of the parasite in the environ­ment. however, little is known about t. gondii prevalence in wild animals and birds in china. methods: we conducted the genetic characterization of t. gondii isolated from zoo wild animals and pet birds in fujian province, southeastern china. heart tissues were collected from 45 zoo animals and 140 pet birds . after identified using b1 gene, the genetic diversity of t. gondii isolates were typed at 11 genetic markers, including sag1,5’ and3’-sag2, alternative sag2, sag3, btub, gra6, c22-8, c29-2, l358, pk1, apico, and cs3. results: seven of 45 zoo animals and 3 of 140 pet birds were positive by pcr amplifi­cation using t. gondii b1 gene specific primers. of these positive isolates, 3 isolates from black-capped ( cebus apella ), peacock ( peafowl ) and budgerigar ( melopsitta­cus undulatus ) were successfully genotyped at 11 genetic loci, and grouped to three distinct genotypes: toxodb genotype #9, #2 and #10, respectively. conclusion: this is the first genotyping of t. gondii isolated from zoo wild animals and pet birds in fujian, china. there is a potential risk for the transmission of this parasite through zoo wild animals and pet birds in this region.